高通量活細(xì)胞高分辨光片顯微鏡

——長(zhǎng)時(shí)間、高通量、活細(xì)胞光片成像系統(tǒng)

      瑞士Viventis公司推出的高通量活細(xì)胞高分辨光片顯微鏡LS系列，是一款全新的光片成像平臺(tái)，主要用于活性的光敏感樣品（如卵子、胚胎、類器官等）的低光毒性、高分辨率的長(zhǎng)期成像。

      高通量活細(xì)胞高分辨光片顯微鏡是近些年發(fā)展起來(lái)的一種創(chuàng)新的成像技術(shù)，它的照明光是一張與成像面平行的薄薄的光片，只有焦平面的樣品被照亮，而光片上下的樣品不受影響。該成像系統(tǒng)在細(xì)胞與組織層面的實(shí)時(shí)成像對(duì)于深入理解生物學(xué)行為至關(guān)重要。尤其適合對(duì)直徑達(dá)300 μm的光敏樣品（如卵母細(xì)胞，胚胎和類器官）進(jìn)行長(zhǎng)期實(shí)時(shí)高時(shí)空分辨率和低光毒性的觀察與成像。

★  雙側(cè)照明光片顯微鏡

      雙側(cè)照明均可以通過(guò)軟件進(jìn)項(xiàng)控制，僅需要點(diǎn)擊鼠標(biāo)就可以控制光束的平移和旋轉(zhuǎn)。光片厚度僅為1.5~6μm，且厚度可調(diào)、位置可自動(dòng)校準(zhǔn)，以適應(yīng)更多的樣本尺寸。配合高NA物鏡，可以實(shí)現(xiàn)更好的穿深，更少的偽影。另外，系統(tǒng)配置可見(jiàn)激發(fā)激光器，通過(guò)檢測(cè)物鏡用戶可對(duì)自定義樣品中感興趣的區(qū)域進(jìn)行快速定位成像操作。

★  軟件界面簡(jiǎn)潔、易于上手

      Viventis系統(tǒng)對(duì)于光片成像初學(xué)者來(lái)說(shuō)操作簡(jiǎn)單，多種模式一鍵切換，軟件界面簡(jiǎn)潔，可以幫助您快速開(kāi)啟光片成像之旅，打開(kāi)lightsheet大門(mén)，助力科研之路。

★  高通量，多樣品同時(shí)成像

      Viventis光片顯微鏡可以快速對(duì)多個(gè)樣品進(jìn)行同時(shí)成像而無(wú)需更換樣品，支持絕大多數(shù)胚胎樣品并可并排擺放，方便添加培養(yǎng)基、加藥等操作。長(zhǎng)工作距離（樣品槽尺寸>50mm），同時(shí)系統(tǒng)可記錄多個(gè)位點(diǎn)并連續(xù)采集。

      對(duì)于細(xì)胞球、類器官等本身較易漂浮的樣本，Viventis也提供了較好的解決方案，采用了人工基底膜/水凝膠嵌入式等方案，實(shí)現(xiàn)上述樣本的穩(wěn)定成像。

腫瘤顯微組織(成纖維細(xì)胞腫瘤細(xì)胞共培養(yǎng))

測(cè)試數(shù)據(jù)

腸道類器官發(fā)育

腫瘤顯微組織(成纖維細(xì)胞腫瘤細(xì)胞共培養(yǎng))

斑馬魚(yú)發(fā)育成像

發(fā)表文章

2023

• Harasimov et al., Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes

Nature Cell Biology

• Olivetta et al., The nuclear to cytoplasmic ratio drives cellularization in the close animal relative Sphaeroforma arctica

bioRvix

2022

• Ozelci et al., Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation.

Nature Communications

• Ishihara et al., Topological morphogenesis of neuroepithelial organoids.

Nature Physics

• de Medeiros et al., Multiscale light-sheet organoid imaging framework

Nature Communication

• Naganathan et al., Left-right symmetry of zebrafish embryos requires somite surface tension.

Nature

• So et al., Mechanism of spindle pole organization and instability in human oocytes.

Science

• Knoblochova et al., CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity.

bioRvix

• Pelzer et al., Ectopic activation of the polar body extrusion pathway triggers cell fragmentation in preimplantation embryos.

bioRvix

2021

• Yang et al., Cell fate coordinates mechano-osmotic forces in intestinal crypt formation.

Nature cell Biology

• He et al., Lineage recording in human cerebral organoids

Nature Methods

• Mailand et al.,Tissue Engineering with Mechanically Induced Solid-Fluid Transitions.

Ad. Materials

• Blengini et al., Aurora kinase A is essential for meiosis in mouse oocytes.

Plos Genetics

• Rohde et al., Cell-autonomous generation of the wave pattern within the vertebrate segmentation clock

bioRvix

2020

• Rossi et al., Embryonic organoids recapitulate early heart organogenesis.

Cell Stem Cell

2019

• Serra et al., Self-organization and symmetry breaking in intestinal organoids development.

Nature

• Dumortier et al., Fracking and Ostwald ripening position the lumen of the mouse blastocyst.

Science

• Welling et al., Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins.

Elife

• Arribat et al., Mitochondria in Embryogenesis: An Organellogenesis Perspective.

Frontiers in Cell and Developmental Biology

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